TABLE 1.
Selected plasmids used in this study and relevant genotypes
| Plasmid | Size (kb) | Relevant genotype | Reference |
|---|---|---|---|
| pBRmSSB | 4.6 | oriE1 ssb tetA | This work |
| pCV546 | 4.3 | oriE1 ssb aph zeo | This work |
| pKD3 | 2.8 | oriR6Kγ FRT-cat-FRT bla | 7 |
| pKD46 | 6.1 | ori101 ParaB-gam-bet-exo repAtspar bla | 7 |
| pCP20 | 9.4 | ori101 cI857 PλpR-flp repAtspar bla cat | 7 |
| pJG9 | 7.0 | ori101 sacB repAtspar cat | 41 |
| pJG9-SSB | 8.8 | ori101 ssb gfpuv sacB repAtspar cat | This work |
| pGEN222 | 6.2 | ori15A gfpuv par bla hok-sok parA | 15 |
| pGEN222S | 7.0 | ori15A gfpuv par ssb bla hok-sok parA | This work |
| pGEN222SXbaI | 7.0 | ori15A gfpuv par ssb bla hok-sok parA | This work |
| pGEN222TS | 6.6 | ori15A gfpuv par ssb FRT-tetA-FRT parA | This work |
| pGEN222S2 | 5.3 | ori15A gfpuv par ssb FRT parA | This work |
| pGEN206 | 7.1 | ori101 gfpuv repA par bla hok-sok parA | 35 |
| pGEN206ΔSpeI | 7.1 | ori101 gfpuv repA par bla hok-sok parA | This work |
| pGEN206S | 7.9 | ori101 gfpuv repA par ssb bla hok-sok parA | This work |
| pGEN206SXbaI | 7.9 | ori101 gfpuv repA par ssb bla hok-sok parA | This work |
| pGEN206TS | 7.5 | ori101 gfpuv repA par ssb FRT-tetA-FRT parA | This work |
| pGEN206S2 | 6.2 | ori101 gfpuv repA par ssb FRT parA | This work |
| pSEC91 | 7.6 | ori15A clyA tetA par aph hok-sok parA | 13 |
| pSEC91SΔclyA | 4.6 | ori15A par ssb FRT parA | This work |
| pSEC91-83 | 8.5 | ori15A clyA::pa83 par aph hok-sok parA | 12 |
| pGEN222TS-83 | 9.0 | ori15A clyA::pa83 par ssb FRT-tetA-FRT parA | This work |
| pSEC91S-83 | 7.7 | ori15A clyA::pa83 par ssb FRT parA | This work |
| pGEN206TS-83 | 10.0 | ori101 clyA::pa83 repA par ssb FRT-tetA-FRT parA | This work |
| pSEC10S-83 | 8.7 | ori101 clyA::pa83 repA par ssb FRT parA | This work |