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. 2009 Oct 19;78(1):518–526. doi: 10.1128/IAI.00257-09

FIG. 5.

FIG. 5.

nsGPLs bind CHO cells expressing the mannose receptor (MR), and the binding is blocked by mannan. CHO cells transfected with the human mannose receptor (CHO-K1-MR) or mock-transfected CHO cells (CHO-K1-V) were incubated with lipid-coated beads for 1 h at a ratio of 50 beads/cell. (A) Quantification by fluorescence microscopy of the number of CHO cells containing attached/phagocytosed FITC-labeled beads coated with nsGPL, ManLAM, or PC. Three separate experiments were conducted, error bars represent SEM, and asterisks indicate statistical significance at P ≤ 0.05 when comparing CHO-K1-MR and CHO-K1-V for corresponding lipid-coated beads. (B) CHO-K1-MR cells incubated in the presence or absence of 2.5 mg/ml mannan were exposed to nsGPL-coated beads for 1 h at a ratio of 50 beads/cell. The number of beads attached/phagocytosed by the CHO cells was quantified by fluorescence microscopy. Three separate experiments were conducted, error bars represent SEM, and asterisks indicate statistical significance at P < 0.0001.