FIG. 3.

Identification of factor H and FHL-1 binding proteins expressed among borrelial isolates. Protein extracts (30 μg each) obtained from B. burgdorferi LW2, B. afzelii FEM1-D15, B. garinii G1, and B. spielmanii PC-Eq17, TIsar2, TIsar3, and A14S were separated by 10% Tris-Tricine-SDS-PAGE and transferred to nitrocellulose. The membrane was incubated with purified factor H (2.5 μg/ml), and binding of the proteins was detected with MAb VIG8 specific for SCR 20 of factor H. For detection of FlaB as a control, MAb L41 1C11 was applied. The identified CRASP proteins are indicated on the right, and the mobility of the marker proteins is indicated on the left.