FIG. 8.

Mapping of the domains of CFH responsible for binding to Erp homologs of B. spielmanii. Schematic representation of the CFH protein and CFH deletion constructs (A). Purified recombinant Erps were separated by 10% Tris-Tricine-SDS-PAGE and transferred to nitrocellulose. The membrane strips were incubated with either several constructs of CFH consisting of SCR domains 1 to 5 (CFH1-5), CFH1-6, CFH1-7 (which represents CFHL1), CFH8-20, CFH15-20, CFH19-20, or CFH15-19 or with NHS as a source of CFH (B). Bound proteins were visualized using MAb B22 specific for SCR5 of CFH and CFHL1, MAb VIG8 specific for SCR20 of CFH, or a polyclonal CFH antiserum for detection of bound SCR15-19. GST fusion proteins were detected by using an anti-goat GST antibody. α, anti.