FIG. 4.

Role of PI3K in M. leprae cytokine induction. Monocytes from healthy donors were pretreated with the PI3K inhibitor LY294002 (LY) or vehicle (0.1% DMSO [D]) or were left untreated (−) for 60 min, followed by stimulation with M. leprae or BCG at an MOI of 5:1 (bacilli to monocyte). Cell supernatants were collected at 24 h postinfection, and cytokines were analyzed by Luminex assay. Values are expressed as percentage activity relative to the M. leprae- or BCG-stimulated untreated cells. Results are the means ± standard error of 10 experiments (representing 10 independent donors) performed in duplicate. A two-tailed paired t test was used for statistical analysis (*, P < 0.05, compared to the untreated M. leprae- or BCG-stimulated cells).