FIG. 4.

Saps are not responsible for the cytokine modulation effect. (A) Human PBMCs were stimulated with heat-killed C. albicans cells in the presence of RPMI or conditioned medium from wild-type (WT) C. albicans or from C. albicans strains defective in different Saps: Δsap1-3, Δsap4-6, or Δsap9-10. Supernatants were collected for IL-6 and IL-8 measurements after 24 h of stimulation. (B) Human PBMCs were stimulated with heat-killed C. albicans cells in the presence of different fractions of C. albicans conditioned medium. Supernatant collected for IL-6 and IL-8 measurements after 24 h shows the relative cytokine percentages induced by conditioned medium above control stimulation with heat-killed C. albicans cells alone.