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Kinetics of formation of membrane pores in human PMNs induced by USA300 supernatant and USA300 Δpvl supernatant. Strains were grown for 18 h in CCY medium as described in Materials and Methods. The diluted supernatants (1:50) were added to leukocyte suspensions and incubated the times indicated. The formation of membrane pores in PMNs was determined by uptake of PI.
