TABLE 1.
Primer sequences and bacterial strains used in this study to produce recombinant toxins
| Toxin | Strain | Source or reference | Primer pair sequencea (5′-3′) |
|---|---|---|---|
| LukS-PVH | LY19990053 | This study | TGGTACTGGCGGCCGCGAAGGTAAAATAACACCAGTC |
| ACGCGGATCCTCAATAATGTCCTTTCACTTTAATTTC | |||
| LukS-PVR | LAC | 26 | TGGTACTGGCGGCCGCGAAGGTAAAATAACACCAGTC |
| ACGCGGATCCTCAATAATGTCCTTTCACTTTAATTTC | |||
| LukF-PV | ATCC 49775 | 23 | ACCCTTAATTAAAGCTCAACATATCACACCTGTAAG |
| ACGCGGATCCTTAGCTCATAGGATTTTTTTCCTTAG | |||
| LukD | A870555 | This study | ACCCTTAATTAAAGCTCAAAATATCACACCTAAAAG |
| ACGCGGATCCTTATACTCCAGGATTAGTTTCTTTAG | |||
| Hlg-B | ATCC 49775 | 23 | TGGTACTGGCGGCCGCGAAGGTAAAATAACACCAGTC |
| ACGCGGATCCTCAATAATGTCCTTTCACTTTAATTTC |
a
The restriction enzymes used for LukS-PV and HlgB cloning were NotI and BamHI, whereas PacI and BamHI were used for LukF-PV and LukD.