FIG. 2.

The absence of BosR results in differential growth of JH211 lacking IPTG under anaerobic and microaerobic conditions. All cultures were grown in triplicate, and cell densities were averaged (bars indicate standard errors). The values shown on the y axis are multiplied by 10⁴/ml to give the final culture density. (A) Strain JH211 was grown under microaerobic conditions (3.48 ppm dissolved O₂, 1% CO₂) without the addition of IPTG (open circles) or with 3 mM IPTG (dark squares). Microaerobic growth of strain A3-LS is also shown (dark triangles). (B) Strain JH211 was grown under anaerobic conditions (0.087 ppm dissolved O₂, 5% CO₂) without IPTG (open circles) or with 3 mM IPTG (dark squares). Asterisks indicate significant differences in growth density between JH211 grown without added IPTG and that grown with 3 mM IPTG added to the growth medium for the day shown (P < 0.05).