FIG. 2.

A single bacterium per vacuole is important for the intracellular proliferation of Salmonella. (A) Percentages (log₁₀) of infected RAW 264.7 cells that have SCVs containing more than three bacteria per vacuole (multiple bacteria per SCV). The graph represents the mean percentages obtained from three experiments. The error bars represent standard errors. Confocal laser scanning microscope images of at least 50 infected cells were analyzed in each case. (B) Fold multiplication of Salmonella inside RAW 264.7 cells. The fold multiplication was calculated by dividing the intracellular bacterial load at 10 h postinfection by the intracellular bacterial load at 2 h postinfection. The graph represents the mean values obtained from an experiment with samples in triplicate. The error bars represent standard errors. *, P < 0.05 (Student's t test).