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. 2009 Oct 19;78(1):348–357. doi: 10.1128/IAI.00721-09

TABLE 1.

Oligonucleotide primers used in this study

Primer Sequence (5′ → 3′) Target region in D39 pneumococcal genome (reference)d
SPD0065F CTGCTAAACTGCCCAGAGATG 65426-67226
SPD0065R GGATACGCAACGTAAGGGAA
SPD0562F CATGGCAAGGTTGAAACTCA 580628-582435
SPD0562R GCCGTGAACGCTATAAGGCGC
SPD0065CF GCGAGATCTTGATGACACGATTTGAGATACGAGAT NAa
SPD0065CF GCGAGATCTTGTCATAAGTTTTCCCCCTTTATATGTT
MALF GCTTGAAAAGGAGTATACTT NAb
PCEPR AGGAGACATTCCTTCCGTATC
MP127 CCGGGGACTTATCAGCCAACC pR410 and pR412 (32)
MP128 TACTAGCGACGCCATCTATGTG
SPD0709F TCGTGTGGCTGCCAAGCGTG 724517-724650
SPD0709R GGCTGATCCACCAGCTGAGTC
SPD0065RTF GGACCTCTTTGTAACAGGAA 66449-66608
SPD0065RTR CATCTGCCAATTCCTTAGGA
SPD0562RTF CCTTAGAGCTAAGCATCCAA 581545-581721
SPD0562RTR GTTGCTGTTTTCCCCCAA
SPD0065IFF TAAGGCCTCTGTCGACACACGATTTGAGATACGAGAc 65847-67631
SPD0065IFR CAGAATTCGCAAGCTTTCATAAGTTTTCCCCCTTTA
a

NA, not applicable.

b

MALF and PCEPR have recognition sequence in pCEP (13).

c

The target region does not include the nucleotides shown in italic, which are complementary to the linearized pEcoli-Nterm 6XHN vector.

d

Annotation is based on reference 27 unless otherwise indicated.