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. 2009 Nov 2;78(1):88–99. doi: 10.1128/IAI.00985-09

FIG. 4.

FIG. 4.

RAW 264.7 cell monolayers infected with B. mallei tssE mutants do not exhibit MNGC formation. Monolayers infected with B. mallei SR1A (pBHR1-TG) (A and B), BM0739 (pA0739G) (C), or BM0739 (pBHR1-TG) (D, E, and F) were fixed at 12 (A and D) or 24 h (B, C, E, and F) postinfection, stained, and examined by DIC and fluorescence microscopy. For panels A to E, monolayers were infected at an MOI 2. For panel F, monolayers were infected at an MOI of 200. Bacteria expressing GFP are shown in green while the nuclei stained with DRAQ5 are shown in blue. Micrographs are representative of at least three independent experiments. Scale bar, 50 μm.