TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequence (5′-3′)a |
|---|---|
| zeo-F | TGTTGACAATTAATCATCGGC |
| EXZeo-E | AGCGGATAACAATTTCACACAGG |
| EXZeo-H | GATTAAGTTGGGTAACGCCAGGG |
| tssE-FKp | CATGGGTACCGAGCTGTATCTGTTCGGCTGCGTG |
| tssE-RH | CATGAAGCTTAGGGGTTGAGCTGTTCGATCAACGG |
| bsaZ-FKp | CATGGGTACCCTTCACGTCACGTCATGCCGAGCGACACG |
| bsaZ-RH | CATGAAGCTTTGTTGGCTAGTGGTCGTTCCC |
| gfp-upSal | CATGGTCGACCTTTGTTAGCAGCCGGATCC |
| gfp-dnSal | CATGGTCGACCCCCTCTAGAAATAATTTTG |
| gfp-upE | CATGGAATTCCTTTGTTAGCAGCCGGATCC |
| gfp-RMCS | CATGCCATGGAAGCTTGAGCTCGGTACCGGATCCTCTAGATCAGTTGTACAGTTCATCCATGCC |
a
Restriction sites in the linker regions are underlined.