FIG. 5.

Expression of cytadherence genes in M. pneumoniae wild-type and mutant cells. Slot blot analyses with whole-RNA extracts of the M. pneumoniae wild type and different mutant strains. A dilution series of RNA extracts was blotted onto a positively charged nylon membrane and probed with a DIG-labeled riboprobe specific for an internal part of a particular open reading frame. Names of riboprobes are given next to each blot. Signals obtained with 1 μg of RNA are shown. Yeast tRNA and M. pneumoniae chromosomal DNA served as controls. Expression profiles of the genes of the P65 operon are shown (A), and the analyses of cytadherence genes of the HMW gene cluster and the major adhesins are also shown (B). The graphs show the quantification of transcript levels relative to that for the M. pneumoniae wild type. Error bars indicate the standard deviation (based on three independent experiments).