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. 2009 Oct 12;78(1):100–107. doi: 10.1128/IAI.01008-09

FIG. 4.

FIG. 4.

Construction of the BBA05 mutant. (A) Strategy for insertional inactivation of the BBA05 gene. wt, genomic structure of BBA05 and the surrounding regions in wild-type B. burgdorferi. pHX05, suicide plasmid used for generating the BBA05 mutant. Only the relevant portion of the plasmid is shown. BBA05, genomic structure of the BBA05 mutant. Small labeled arrows denote positions of oligonucleotide primers used for PCR analyses. (B) Confirmation of the BBA05 mutant by PCR analyses. Letter combinations denote primer pairs used for PCR. kb, DNA ladder; wt, wild-type strain; BBA05, BBA05 mutant. (C) Expression of BBA04, BBA05, and BBA06 in the wild type and the BBA05 mutant by RT-PCR analyses. Spirochetes were cultured at 35°C and harvested at late logarithmic growth. RT-PCR was performed using primers specific for BBA04, BBA05, or BBA06.