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. 2009 Oct 26;78(1):495–504. doi: 10.1128/IAI.00838-09

FIG. 5.

FIG. 5.

(A) Arrangement of the cpb and tpeL gene locus in C. perfringens type B isolate ATCC 3626 (based upon sequencing results released from JCVI). Solid black bars indicate the span of overlap PCR products. (B) Overlap PCR analysis of the region extending from cpb to tpeL in NCTC 3110 using reaction R1 to R5 primers (Table 2). (C) Long-range PCR assay using an internal cpb primer and an internal tpeL primer to link the tpeL and cpb genes.