FIG. 2.

Iron treatment does not affect the development of pulmonary pathology. BALB/c mice were intranasally infected with approximately 10 LD₅₀ of KIM D27 (1 × 10⁵ CFU) (A to D) or CO92 (3 × 10³ CFU) (E and F). The mice were euthanized when moribund, and lungs were perfused and fixed in 10% formalin prior to sectioning and staining with H&E. (A and B) Lungs from KIM D27-infected mice without iron treatment. Tissues shown are representative of two independent studies (n = 8). (C and D) Lungs from KIM D27-infected mice that were injected with 50 μg of iron intraperitoneally just prior to challenge. Tissues shown are representative of three independent studies (n = 29). (E and F) Lungs from moribund CO92-infected mice (n = 2).