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Bacterial titers in the blood, spleen, and lung show KIM D27 colonization of moribund mice. BALB/c mice (n = 5) were infected intranasally with 1.0 × 107 CFU of KIM D27 (1,000 LD50) and were euthanized when moribund. Tissues were homogenized in sterile PBS and were plated on HIA in triplicate for determination of bacterial loads in the blood, spleen, and lung at the time of euthanasia. Average counts are reported. Symbols with the same shape correspond to the same mouse.
