TABLE 1.
B. subtilis mutant strains
| Strain | Genotypea | Reference |
|---|---|---|
| SSB1044 | mrnC::pMUTIN2 (mrnC::ery) | 15 |
| SSB1030 | pnp::cm | 17 |
| BG457 | rph::spc | 14 |
| BG393b | rph::cm rnr::spc | 17 |
| BG395b | rph::cm yhaM::pm | 17 |
| BG396 | rph::cm rnr::spc yhaM::pm | 1 |
| BG508c | pnp::kan rph::spc rnr::tc | 1 |
| CCB084 | amyE::pCT1[Pxyl-rnjA] Cm rnjA::spc | 2 |
| CCB166 | mrnC::ery amyE::pCT1[Pxyl-rnjA] Cm rnjA::spc | This work |
| CCB167 | mrnC::ery pnp::cm | This work |
| CCB168 | mrnC::ery rnr::spc | This work |
| CCB169 | mrnC::ery rph::spc | This work |
| CCB170 | mrnC::ery yhaM::pm | This work |
| CCB225 | mrnC::ery yhaM::pm rph::cm | This work |
| CCB171 | mrnC::ery rnr::spc rph::cm | This work |
| CCB172 | mrnC::ery rnr::spc rph::cm yhaM::pm | This work |
| CCB173 | mrnC::ery rnr::spc rph::cm yhaM::pm pnp::kan | This work |
a
Resistance markers are as follows: ery, erythromycin; cm, chloramphenicol; kan, kanamycin; pm, phleomycin; and spc, spectinomycin.
b
SSB1044 transformants with these DNAs were screened for Cms to obtain CCB168 and CCB170 double mutants only.
c
CCB172 transformants with DNA from strain BG508 were selected for Eryr, Pmr, Kanr, Cmr, Spcr, and Tcs.