TABLE 1.
Strains and plasmids used in this study
| Strain/plasmid | Descriptiona | Source/reference |
|---|---|---|
| Strains | ||
| Neisseria meningitidis | ||
| MC58 | L3 immunotyping reference strain | 20 |
| MC58 galE | L3 galE | 12 |
| MC58 galE lpt3 | L3 galE lpt3::Emr | 19 |
| 89I | L4 immunotyping reference strain | |
| 89I galE | L4 galE::Emr | 39 |
| Escherichia coli | ||
| DH5α | F− φ80 LacZ M15 (lacZY-argF) supE44 thi-1 gyrA96 recA1 endA1 relA1 hsdR17 | |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen |
| CQW50 | Lpt3-His6 expression strain; BL21(DE3) containing pCQJR3; Kmr | This study |
| CQW51 | Lpt6-His6 expression strain; BL21(DE3) containing pCQJR7; Kmr | This study |
| Plasmids | ||
| pET28a | T7 expression vector, N- and C-terminal His6 tags; 5.4 kb; Kmr | Novagen |
| pET30a | T7 expression vector, N- and C-terminal His6 tags; 5.4 kb; Kmr | Novagen |
| pCQJR3 | Lpt3-His6 expression vector; PCR product of CQ-160 and CQ-162, containing lpt3, cloned into the NdeI and XhoI sites of pET30a; 6.8 kb; Kmr | This study |
| pCQJR7 | Lpt6-His6 expression vector; PCR product of CQ-222 and CQ-223, containing lpt6, cloned into the NcoI and XhoI sites of pET28a; 7.0 kb; Kmr | This study |
a
Em, erythromycin.