FIG. 5.
Segregation of SLP2tsrΔpar in normal and small colonies of strains TK64 and M145. Fifty small colonies and fifty normal-sized colonies of each culture were tested for thiostrepton resistance on R5Cu medium. The presence of the plasmid in the Thior colonies was confirmed by PCR in selected samples. Spores of Thior colonies derived from normal-sized and small colonies were collected and plated on nonselective medium (Progeny), and both small and normal-sized colonies were found in all cases, as exemplified in the photos.