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Pocks caused by conjugal transfer of SLP2tsr and its variants. Serial dilutions of spores of TK64 harboring SLP2tsr or its variants (-Δpar, -Δtra, and -Δspd) were made and spotted on R5 medium seeded with about 108 spores of M145 (top), TK54 (middle), or TK64 (bottom). The plates were incubated at 30°C for 3 to 4 days. Pocks formed around single-donor colonies were photographed. Note that no pocks were visible in the SLP2tsrΔtra samples.
