Skip to main content
. 2009 Oct 23;192(1):316–325. doi: 10.1128/JB.01228-09

FIG. 5.

FIG. 5.

Increased frequency of PilC phase variation in MMC-deficient Gc. (A) Increased colony variation in Gc lacking recA and MMC. FA1090 recA6 Gc and isogenic mutS and mutL mutants and complements were assayed for pilus-dependent colony variations as described for Fig. 4, except bacteria were grown on GCB lacking IPTG to repress recA expression. Statistical significance was calculated as for Fig. 4. The MMC and uvrD mutants exhibited a significantly higher PDCMC score than either the parental strain or its isogenic complement; asterisks indicate P < 0.025 at matched time points (>16 h). (B) Absence of PilC in P− outgrowths isolated from Gc lacking recA and MMC. P+ FA1090 recA6 Gc lacking mutL or mutS were grown on GCB without IPTG to repress recA expression. Lysates from four P− colonies for each strain were isolated (labeled 1 to 4). Lysates from the P− bacteria and FA1090 recA6 (P+) were immunoblotted for expression of the 110-kDa PilC protein.