TABLE 2.
Transformation frequencies to kanamycin resistance in parental and MMC-deficient Gc
| DNA form | Strain | Transformation frequencya | Fold increaseb |
|---|---|---|---|
| Chromosomal | FA1090 | 9.24 (±3.46) × 10−5 | |
| DNA | mutL | 11.8 (±3.09) × 10−5 | 1.3 |
| mutS | 8.30 (±1.62) × 10−5 | 0.9 | |
| Cloned plasmid | FA1090 | 19.3 (±9.03) × 10−5 | |
| DNA | mutL | 29.6 (±7.44) × 10−5 | 1.5 |
| mutS | 19.2 (±7.56) × 10−5 | 1.0 |
a
Number of Kanr CFU/total number of CFU (± SEM).
b
The increase compared to the FA1090 parent. None of the increases in transformation to kanamycin resistance was statistically significant relative to the FA1090 parent.