FIG. 1.

Isolation of V. alginolyticus basal bodies solubilized using CHAPS. (a) Coomassie blue-stained SDS-PAGE of proteins in the purification steps using CHAPS. Lane 1, whole-cell lysate. Lane 2, supernatant fraction from low-speed centrifugation. Lane 3, supernatant fraction from ultracentrifugation. Lane 4, precipitate fraction from ultracentrifugation. (b) The proteins in the purification steps were detected by immunoblotting using anti-MotY, anti-FliG, and anti-FliM antibodies. Fractionated samples were loaded in the same order as in panel a.