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Electrophoretic mobility shift assays of His6-RhaS(163-278) binding to RhaS and RhaR half sites. Electrophoretic mobility shift reactions were carried out in the absence (−) or presence (+) of 6 μM purified His6-RhaS(163-278) with 49-bp 32P-labeled DNA fragments including each of the RhaS half sites at rhaBAD and the RhaR half sites at rhaSR. The direction of electrophoresis was from the top down, as shown. The percent DNA bound was averaged from four independent assays.
