TABLE 2.
Φ(rhaS-lacZ) promoter truncation | ΔrhaSrhaR+ straina |
ΔrhaSR straina |
||||
---|---|---|---|---|---|---|
Avg β-galactosidase activity (Miller units)b in strain carrying: |
Fold repression by RhaS | Avg β-galactosidase activity (Miller units) in strain carrying: |
Fold activation by RhaS | |||
Vector onlyc | rhaS+ plasmidc | Vector only | rhaS+ plasmid | |||
Φ(rhaS-lacZ)Δ85 | 6.4 | 4.4 | 1.5 | 0.17 | 4.5 | 26 |
Φ(rhaS-lacZ)Δ128 | 630 | 14 | 45 | 0.84 | 17 | 20 |
The strain background was either ΔrhaS rhaR+zih-35::Tn10 or Δ(rhaSR)::kan.
β-Galactosidase activity was measured from single-copy lacZ fusion strains grown in MOPS-buffered minimal growth medium containing chloramphenicol and l-rhamnose. The standard errors were less than 30% of the average activities.