. 2009 Oct 23;192(1):225–232. doi: 10.1128/JB.00829-08

TABLE 2.

Repression and activation of rhaSR expression by RhaS

Φ(rhaS-lacZ) promoter truncation	ΔrhaSrhaR⁺ strain^a			ΔrhaSR strain^a
	Avg β-galactosidase activity (Miller units)^b in strain carrying:		Fold repression by RhaS	Avg β-galactosidase activity (Miller units) in strain carrying:		Fold activation by RhaS
	Vector only^c	rhaS⁺ plasmid^c	Fold repression by RhaS	Vector only	rhaS⁺ plasmid	Fold activation by RhaS
Φ(rhaS-lacZ)Δ85	6.4	4.4	1.5	0.17	4.5	26
Φ(rhaS-lacZ)Δ128	630	14	45	0.84	17	20

The strain background was either ΔrhaS rhaR⁺zih-35::Tn10 or Δ(rhaSR)::kan.

β-Galactosidase activity was measured from single-copy lacZ fusion strains grown in MOPS-buffered minimal growth medium containing chloramphenicol and l-rhamnose. The standard errors were less than 30% of the average activities.

The vector was pSU18 (2). RhaS was expressed from plasmid pSE273 (34), which is pSU18 rhaS⁺.