TABLE 3.
Comparison of transcription and translation of the rhaS and rhaR genes
| Fusion type | Φ(rhaS-lacZ) promoter truncationa | Avg β-galactosidase activity (Miller units)b |
|
|---|---|---|---|
| − Rha | + Rha | ||
| Transcriptional | Φ(rhaS-lacZ)(−128 → +84) | 20 | 1,330 |
| Φ(rhaSR-lacZ)(−128 → +904) | 26 | 1,540 | |
| Translational | Φ(rhaS-lacZ)(−128 → +84) | 0.04 | 170 |
| Φ(rhaSR-lacZ)(−128 → +904) | 0.02 | 6.0 | |
a
The strain background was E. coli ECL116 for all promoter truncations shown (1).
b
β-Galactosidase activity was measured from single-copy lacZ fusion strains grown in MOPS-buffered minimal growth medium with or without l-rhamnose, as indicated. The standard errors were less than 8% of the average activities, except for values less than 1.0, where standard error was less than 25%.