FIG. 2.

In vivo functional analysis of class M CobT variant proteins. Strains were grown in NCE minimal medium supplemented with glycerol and Cbl (positive control) or Cbi with or without DMB (for details, see Materials and Methods). The chromosomal copies of the cobB and recA genes in strain JE2607 (cobT cobB recA) were inactivated to eliminate nonspecific catalysis of the CobT reaction by the CobB sirtuin and to prevent homologous recombination between the plasmid and chromosomal alleles of cobT.