FIG. 5.

Phenotypic analyses of strains that synthesize class M mutant CobT proteins with putative compensatory substitutions. We tested in vivo the ability of class M variant CobT proteins with putative compensatory substitutions to restore Cbl synthesis in strain JE2607 (cobT cobB recA1). Conditions for the assessment of growth on ethanolamine as a carbon and energy source were as described in Materials and Methods. All of the media tested contained (CN)₂Cbi and DMB (600 μM); CNCbl was used as a positive control.