TABLE 1.
Bacterial strains and vectors used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or referencea |
|---|---|---|
| A. hydrophila strains | ||
| SSU | Diarrheal isolate of A. hydrophila | CDC |
| SSU-R | Rifampin-resistant (Rifr) strain | Laboratory stock |
| Δact strain | Isogenic act gene mutant of SSU-R; Rifr Kmr | 14 |
| Δact ΔvasH strain | Isogenic vasH gene mutant of SSU-R Δact; Rifr Kmr Smr/Spr | 44 |
| Δact ΔvasH vasH strain | Isogenic vasH gene mutant of SSU-R Δact strain complemented with vasH in pBR322; Rifr Kmr Smr/Spr Apr | This study |
| Δact vgrG1::bla strain | Isogenic act gene mutant of SSU-R expressing the vgrG1::bla gene in pGEN222; Rifr Kmr Apr | This study |
| Δact vgrG1-NH2::bla strain | Isogenic act gene mutant of SSU-R expressing the vgrG1-NH2::bla gene in pGEN222; Rifr Kmr Apr | This study |
| Δact ΔvasH vgrG1::bla strain | Isogenic vasH gene mutant of SSU-R Δact strain expressing the vgrG1::bla gene in pGEN222; Rifr Kmr Smr/Spr Apr | This study |
| Δact ΔvasH vgrG1-NH2::bla strain | Isogenic vasH gene mutant of SSU-R Δact expressing the vgrG1-NH2::bla gene in pGEN222; Rifr Kmr Smr/Spr Apr | This study |
| ATCC 7966 | Environmental isolate | ATCC |
| E. coli strains | ||
| DH5α | Production of recombinant plasmids; recA gyrA | Life Technologies |
| HMS174-DE3 | Production of recombinant proteins cloned in pET-30a, carries a T7 RNA polymerase copy under the control of lacUV5 promoter | Novagen |
| Plasmids | ||
| pET-30a | pBR322-derived expression vector with T7 lac promoter, expression and production of recombinant proteins with 6×His tag; Kmr | Novagen |
| pBI-EGFP | Expression and production of recombinant proteins in Tet-Off eukaryotic system; Apr | Clontech |
| pBR322 | Apr Tcr | GE Healthcare |
| pGEN222 | Apr | 11 |
a
CDC, Centers for Disease Control and Prevention; ATCC, American Type Culture Collection.