TABLE 3.
Mass spectrometric alignment of differentially produced protein spots from supernatants of A. hydrophila SSU Δact and Δact ΔvasH strains, based on 2-D gel electrophoresisa
| Spot | Rank | Protein with highest homology | Protein expectation score |
|---|---|---|---|
| 5_4804 | 1 | VgrG3 protein of Aeromonas hydrophila | 9.97631E-70 |
| 5_5573 | 1 | VgrG3 protein of Aeromonas hydrophila | 2.50594E-65 |
| 5_5562 | 1 | VgrG2 protein of Aeromonas hydrophila | 9.97631E-53 |
| 5_4798 | 1 | VgrG3 protein of Aeromonas hydrophila | 6.29463E-46 |
| 5_6426 | 1 | VgrG3 protein of Aeromonas hydrophila | 3.15479E-43 |
| 5_4785 | 1 | VgrG3 protein of Aeromonas hydrophila | 6.29463E-39 |
| 3_3026 | 1 | Putative hemolysin-coregulated protein of Aeromonas hydrophila | 1.99054E-35 |
| 5_4809 | 1 | Rhselement Vgr family protein ofAeromonas hydrophilasubsp.hydrophilaATCC 7966 | 3.97164E-34 |
| 5_5996 | 1 | VgrG3 protein of Aeromonas hydrophila | 3.97164E-33 |
| 5_6503 | 1 | Putative VgrG protein of Aeromonas hydrophila | 9.97631E-26 |
| 5_5611 | 1 | Phosphatidylcholine-sterol acyltransferase of Aeromonas hydrophila subsp. hydrophila ATCC 7966 | 9.97631E-20 |
| 5_6093 | 1 | Putative VgrG protein of Aeromonas hydrophila | 7.92447E-14 |
| 3_5936 | 1 | Rhselement Vgr family protein ofAeromonashydrophilasubsp.hydrophilaATCC 7966 | 1.25594E-13 |
| 5_5992 | 1 | VgrG3 protein of Aeromonas hydrophila | 9.97631E-10 |
| 3_4252 | 1 | HcpA homolog of Aeromonas hydrophila subsp. hydrophila ATCC 7966 | 9.97631E-09 |
| 3_6571 | 1 | HcpA homolog of Aeromonas hydrophila subsp. hydrophila ATCC 7966 | 1.99054E-08 |
| 5_5746 | 1 | Hypothetical protein AHA_3948 of Aeromonas hydrophila subsp. hydrophila ATCC 7966 | 1.25594E-05 |
| 5_6471 | 1 | Inorganic pyrophosphatase of Aeromonas hydrophila subsp. hydrophila ATCC 7966 | 0.007924466 |
| 5_4403 | 1 | VgrG protein of Aeromonas salmonicida subsp. salmonicida A449 | 0.019905359 |
| 5_539 | 1 | Type III restriction protein Res subunit of Xanthobacter autotrophicus Py2 | 0.019905359 |
| 5_260 | 1 | Ribosomal large subunit pseudouridine synthase B of Aeromonas salmonicida subsp. salmonicida A449 | 0.039716412 |
| 5_4807 | 1 | Transcriptional regulator of GntR family of Mesorhizobium sp. BNC1 | 0.199053585 |
| 3_6711 | 1 | Hypothetical protein ECA4275 of Erwinia carotovora subsp. atroseptica SCRI1043 | 0.629462706 |
| 3_6570 | 1 | Hypothetical protein Acry_2197 of Acidiphilium cryptum JF-5 | 1.255943216 |
| 5_5609 | 1 | Flagellar hook-associated 2 domain protein of Shewanella baltica OS155 | 1.255943216 |
| 5_6215 | 1 | Hypothetical protein msr9585 of Mesorhizobium loti MAFF303099 | 1.255943216 |
| 5_4784 | 1 | Hypothetical protein RPC_0976 of Rhodopseudomonas palustris BisB18 | 2.505936168 |
| 5_5356 | 1 | Regulatory protein of Oceanicola granulosus HTCC2516 | 6.294627059 |
| 5_548 | 1 | Hypothetical protein HI0688 of Haemophilus influenzae Rd KW20 | 6.294627059 |
| 5_6707 | 1 | Peptidyl-prolyl cis-trans isomerase C of Yersinia pestis KIM | 6.294627059 |
| 5_6113 | 1 | Putative ABC-type multidrug transport system, ATPase component, of Photobacterium profundum 3TCK | 7.924465962 |
| 5_6708 | 1 | General secretion pathway protein L of Vibrio cholerae MZO-3 | 15.8113883 |
a
Data in bold font represent protein spots matching VgrG1 from A. hydrophila ATCC 7966.