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Bacterial localization of the InhA2 and InhA3 proteins. Strains 407 ΔinhA1 ΔinhA3 and 407 ΔinhA1 ΔinhA2 were grown on LB medium and were harvested at t2 and t4. Supernatant (Sn) and membrane (Mb) fractions were blotted with a rabbit polyclonal antibody recognizing the different InhA proteins, and the specific locations of InhA2 and InhA3 were determined.
