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Predicted prion-prone regions. The PFD of Sup35-27 (A) or wild-type Sup35 (B) was scanned using an 11-amino-acid window size. At each position within the prion domains, the sum of ln (ORobs) for the indicated amino acid and the five amino acids on either side was calculated to determine the prion propensity of the window. Regions mutated in libraries 1 and 2 are indicated.
