TABLE 3.
Physical properties of prion-forming isolates from library 1
| Property | Mean value ± SEM for naive library | Mean value ± SEM for prion-forming isolates | P value |
|---|---|---|---|
| Hydrophobicitya | −4.30 ± 0.72 | 0.02 ± 0.75 | 1.8 × 10−4 |
| β-Sheet propensityb | 3.18 ± 0.10 | 2.34 ± 0.09 | 3.5 × 10−7 |
| α-Helix propensityc | 1.35 ± 0.17 | 0.73 ± 0.18 | 0.028 |
| Charged | 0.88 ± 0.11 | 0.44 ± 0.12 | 0.016 |
a
From reference 39. Higher values represent greater hydrophobicities. For each naive or prion-forming isolate, the sum of the hydrophobicities of each residue within the mutagenized region was calculated. Data are the average sum per construct for the respective libraries. Standard errors are indicated.
b
From reference 48. Higher values represent lower β-sheet propensities.
c
From reference 22. Higher values represent lower α-helix propensities.
d
The absolute value of the net charge of the mutated region.