FIG. 6.

An N-terminal domain required for Dia2 protein turnover overlaps the NLS region. (A) Addition of the SV40 T antigen NLS to Dia2 N-terminal deletions restores nuclear localization. Immunofluorescence was performed as for Fig. 4. Scale bar, 5 μm. (B) Nuclear, N-terminal Dia2 deletions are stable. Dia2 mutants were subjected to stability assays as described for Fig. 4. Quantitation of results from three independent experiments is shown on the graph. Error bars indicate standard deviations. (C) Overexpression of stable, nuclear forms of Dia2 alters cell cycle distribution. Wild-type cells expressing the indicated DIA2 alleles from a galactose-inducible promoter were grown to log phase in galactose-containing medium. DNA content was measured by flow cytometry. Quantitation of results from three independent experiments in shown in the graph.