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. 2009 Oct 26;30(1):344–353. doi: 10.1128/MCB.00444-09

FIG. 3.

FIG. 3.

JMJD1A regulates hypoxic induction of a subset of hypoxia-regulated genes in RCC4+VHL cells. (A) Quantitative RT-PCR of JMJD1A expression in RCC4+VHL cells transfected with siRNA to JMJD1A (siD1A) and exposed to 21% or 0.5% oxygen for 16 h. (B) Venn diagram graphically showing the intersection between genes induced greater than 1.5-fold by hypoxia in the siControl-transfected cells (left circle) and genes reduced at least 1.5-fold under hypoxia with siJMJD1A compared to the hypoxic siControl (right circle). Genes dependent on JMJD1A for hypoxic induction are depicted in the overlap. (C) RCC4+VHL cells were transfected as for panel A, and the expression of genes identified in panel B (ADM, SERPINE1, EDN1, and HMOX) was measured by QRT-PCR. STC2 and PFKFB3 serve as examples of two JMJD1A-independent hypoxia inducible genes. Data are from six experiments measured in triplicate (± standard errors of the means) and normalized to the normoxic control siRNA (SiCon). Statistical significance was calculated using Student's t test: *, P < 0.05; **, P < 0.01.