FIG. 6.

JMJD1A promotes tumor growth. (A) Loss of JMJD1A reduces tumor growth rate. A total of 3 × 10⁶ HCT116 cells expressing shRNA to JMJD1A (shD1A-2; n = 3) or a nonsilencing control (NSC; n = 5) were injected into the flanks of SCID mice and the volume measured at the indicated intervals. Data are the averages of tumor volumes ± standard errors of the means. *, P < 0.05 as determined by nonlinear regression. (B) Loss of JMJD1A has no effect on in vitro growth rate. A total of 10⁵ HCT116 cells expressing shRNA to JMJD1A or a nonsilencing control were grown as described in Materials and Methods in 21% oxygen or 0.5% oxygen. NS indicate a nonsignificant difference in growth rate as measured by nonlinear regression. (C) Hypoxic JMJD1A expression is abolished in HIF-1α knockout (HKO) HCT116 cells exposed to 21% and 0.5% O₂ for 16 h. Expression is normalized to that in normoxic wild-type cells (WT). (D) Box-and-whisker plot of JMJD1A expression in renal cell carcinoma. The plot is a scalable vector graphic derived from a microarray analysis by Gumz et al. (14) downloaded from Oncomine (http://www.oncomine.org/) with text labels modified for clarity.