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Glc7 is not involved in the recovery from MMS-induced DNA damage. Exponentially growing wild-type (wt), glc7-129, glc7-132, and glc7-T152K cells were arrested in G1 with α-factor (αf) and released from the pheromone block in YPD or YPD containing 0.005% MMS. Aliquots of each culture were harvested at the indicated times after α-factor release to determine DNA content by FACS analysis (A) and to detect Rad53 by Western blot analysis with anti-Rad53 antibodies (B).
