FIG. 7.

Lack of γH2A formation alleviates the recovery defects of HU-treated glc7 mutants. (A) Serial dilutions (1:10) of exponentially growing cultures of wild-type (wt), glc7-T152K, hta2-S129A, and glc7-T152K hta2-S129A cells were spotted onto YPD plates with or without HU or phleomycin at the indicated concentrations and incubated at 25°C for 3 days. (B and C) Cells were released from G₁ arrest (αf) in YPD medium with 0.03 M HU. Aliquots were harvested at the indicated times after release to determine DNA content by FACS analysis (B) and to detect Rad53 by Western blot analysis (C). The hta2-S129A and glc7-T152K hta2-S129A strains also carry the HTA1 deletion.