FIG. 8.

Epistatic relationships between GLC7, PPH3, PTC2, and PTC3. (A) Serial dilutions (1:10) of exponentially growing cells with the indicated genotypes were spotted onto YPD plates with or without MMS, HU, and phleomycin at the indicated concentrations and incubated at 25°C for 3 days. wt, wild type. (B and C) Exponentially growing cells with the indicated genotypes were arrested in G₁ with α-factor (αf) and released from the pheromone block in YPD or in YPD containing 0.03 M HU. Aliquots of each culture were harvested at the indicated times after α-factor release to determine DNA content by FACS analysis (B) and to detect Rad53 by Western blot analysis with anti-Rad53 antibodies (C).