FIG. 8.

Phosphorylation-dependent cofactor-binding increases AF1-mediated transcriptional activity of a promoter containing 3×GRE. (A) S211A mutant inhibits GRE-mediated AF1 activity, as assessed by SEAP-based promoter-reporter assay in CV-1 cells. (B) Treatment of pharmacological inhibitors of p38 (SB203580 or SB202190) inhibits GRE-mediated GR AF1 activity in a promoter-reporter assay. SB1, SB203580; SB2, SB202190. The results are expressed as means ± the standard error. Experiments were repeated five times. The levels of significance were evaluated by a two-tailed paired Student t test, and a P value of <0.05 was considered significant. Graphs were normalized to the transfection efficiency of each construct assayed by immunoblotting with specific antibodies for GR, TBP, CBP, SRC-1, and GFP (data not shown).