TABLE 2.
Summary of changes in the relative fluorescence intensity and wavelength maximum after AF1 phosphorylationa
| Parameter | Change in relative fluorescence intensity |
|||
|---|---|---|---|---|
| AF1 | AF1-P | AF1S211A | AF1S211A-P | |
| F/F0 max (278 nm) | 5.31 | 7.55 | 5.08 | 5.50 |
| λmax (278 nm) | 351 | 346 | 352 | 352 |
| F/F0 max (295 nm) | 4.22 | 5.71 | 4.28 | 4.17 |
| λmax (295 nm) | 355 | 341 | 357 | 356 |
a
Values were calculated from fluorescence data obtained at a 278- or 295-nm excitation wavelength (Fig. 4). F/F0, relative fluorescence intensity at λmax; λmax, the wavelength at the maximum fluorescence intensity. AF1, unphosphorylated AF1; AF1-P, phosphorylated AF1.