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. 1998 Sep 1;95(18):10842–10847. doi: 10.1073/pnas.95.18.10842

Figure 2.

Figure 2

Determination of apoptosis and clonogenic survival after γ-irradiation of human glioblastoma cells. (A) Cells were plated and allowed to attach before being exposed to γ-irradiation (10 Gy) in 10% serum or serum-free media. After 72 h, quantitation of apoptosis was conducted by two independent observers. The apoptotic index is the percentage of apoptotic cells with morphologic evidence of apoptosis as determined by staining of nuclei with DAPI. Results presented are mean ± SEM of four independent experiments, and the mean is indicated in parentheses. U87MG cells were grown in 10% serum (□) or serum-free media (▧) and U87/T691 cells were grown in 10% serum (▩) or serum-free media (■). (B) U87MG and U373MG human glioma cells and derivatives were stained with DAPI and analyzed for apoptotic morphology 72 h after γ-irradiation. The mean is indicated in parentheses, and the index shown in this representative experiment is mean ± SD. These results are representative of two additional experiments. Apoptotic indices were felt to be an underestimate because floating cells could not be assayed by this technique.