FIG. 4.

Characterization of DR-type RAR binding sites. (A to E) Competition EMSAs. EMSAs were performed using Rarbwt DR5 as a radioactively labeled probe and a 100-fold excess of the unlabeled competitor (Comp) oligonucleotides shown above each lane. The sequences of the DRs within the oligonucleotides are indicated at the right of each panel. Mismatches with the consensus sequence are shown in red. For the chimeric DR, a color code for each half site is used. The results of competition EMSAs and ChIP assays are summarized on the right. (F and G) ChIP-qPCR on the indicated loci using amplicons centered on the DR element.