FIG. 7.

Identification of RAR binding sites in ES cells. (A) Schematic representation of RARs with a C-terminal 3×Flag-SBP or 3×Flag-HA tag and Western blot assay showing the expression of tagged and endogenous RARs in extracts of recombined or control ES cells. (B) Summary of classical DR-type RAR binding motifs at ChIPed ES cell loci as described in the legend to Fig. 1C. (C) Summary of gene ontology analysis of target genes. (D to F) Graphic representation of tandem Flag ChIP-chip results on ES cells and MEFs expressing tagged RARγ in the UCSC web browser at the indicated loci. The y axis shows the normalized immunoprecipitate/input ratio. (G and H) Flag ChIP-qPCR at the indicated loci in tagged and control MEFs and ES cells.