Figure 6.

Characterization of Lhca6.

(A) Phylogenetic tree of Lhca family proteins. Sequences of Lhca5 and Lhca6 were retrieved from GenBank (http://www.ncbi.nlm.nih.gov/). The sequences are named for each organism. The sequences of Lhca1-9 of Chlamydomonas and Lhca1-4 of Arabidopsis were also included in the analysis. The corresponding amino acid sequences were aligned with the ClustalW program with default settings, and an unrooted tree was constructed using TreeView software.

(B) Immunodetection of Lhca6 using a monoclonal antibody against the HA tag. The lhca6 plants were transformed with the Os Lhca6 gene fused to the sequence encoding the HA tag. Wild-type and crr2-2 plants were transformed with At Lhca6 cDNA fused to the region encoding the HA tag expressed under the control of the CaMV 35S promoter. Thylakoid complexes isolated from transformants were separated by BN-PAGE and further subjected to 2D SDS-PAGE. The proteins were immunodetected with specific antibodies. The positions of NDH-PSI supercomplex and PSI monomer are indicated by closed arrows. The position of the putative subsupercomplex detected in crr2-2 is indicated by an open arrow.