Figure 8.

Characterization of the lhca6 pgr5 Double Mutant.

(A) Visible phenotype of the double mutants. Seedlings were cultured at 50 μmol photons m⁻² s⁻¹ for 3 weeks after germination.

(B) High chlorophyll fluorescence phenotype of lhca6 pgr5. Dark-adapted seedlings of the wild type and knockout mutants were illuminated at 100 μmol photons m⁻² s⁻¹ for 1 min and then a chlorophyll fluorescence image was captured by CCD camera. Arrows indicate immature leaves of lhca6 pgr5 emitting less fluorescence than mature leaves.

(C) Light intensity dependence of the relative ETR. ETR is shown relative to the maximum ETR in the wild type (100%); means ± sd (n = 5).

(D) Immunodetection of chloroplast proteins. The thylakoid membrane proteins were separated by SDS-PAGE and immunodetected with antibodies against NdhH, NdhL, and Cytf proteins. Thylakoid proteins were loaded on an equal chlorophyll basis.