Figure 2.

The Experimental Design to Assess the Effects of Light and Temperature on the Development of Variegation in the im Mutant of Arabidopsis.

Seeds from both the wild type and im were germinated and allowed to grow at 25°C under an irradiance of 5 μmol photons m⁻² s⁻¹ (25/5) for 7 d. One group of these seedlings was shifted to either 50 (25/50), 150 (25/150), or 450 (25/450) μmol photons m⁻² s⁻¹ for up to 42 d. In addition, a second group of the same seedlings was shifted to 12°C at an irradiance of 50 (12/50), 150 (12/150), and 450 (12/450) μmol photons m⁻² s⁻¹. All plants were grown with an 8/16-h day/night cycle. Photographs shown are only of im plants.