Skip to main content
. 2009 Nov 4;84(2):867–882. doi: 10.1128/JVI.01571-09

FIG. 3.

FIG. 3.

μNS aa 1 to 12 or aa 14 to 41 are sufficient for associations with the nonstructural protein σNS. For each experiment, cells were processed for fluorescence microscopy at 18 h p.t. (A) CV-1 cells were cotransfected with plasmids expressing σNS and either EGFP/NSP5 (top), μNS(1-12)/EGFP/NSP5 (middle), or μNS(1-20)/EGFP/NSP5 (bottom). After fixation, cells were stained with mouse monoclonal antibody 3E10 against σNS followed by Alexa 594-conjugated goat anti-mouse IgG to visualize σNS (right). The inherent fluorescence of EGFP was used to visualize each of the fusion proteins (left). (B) CV-1 cells were cotransfected with plasmids expressing σNS and either μNS(14-41)/EGFP/NSP5 (top) or μNS(20-41)/EGFP/NSP5 (bottom). After fixation, cells were stained with mouse monoclonal antibody 3E10 against σNS followed by Alexa 594-conjugated goat anti-mouse IgG to visualize σNS (right). The inherent fluorescence of EGFP was used to visualize each of the fusion proteins (left). Bar, 10 μm.